Multiple sequence alignment (MSA) is a cornerstone of computational biology, facilitating the exploration of functional, structural and evolutionary relationships among biological sequences.

The standard pipeline for 16S amplicon analysis starts by clustering sequences within a percent sequence similarity threshold (typically 97%) into ‘Operational Taxonomic Units’ (OTUs). From each OTU, ...

Roestel, Jessica A., Wiersema, John H., Jansen, Robert K., Borsch, Thomas, and Gruenstaeudl, Michael. 2023. [Dataset] On the importance of correct DNA sequence ...